Recombinant Expression of Bornavirus P24 Protein for Enzyme-Linked Immunosorbent Assay Development
Abstract:
BDV is a neurotropic enveloped RNA
virus that induces persistent neurologic disease in a wide
host range, including several vertebrate species and humans. The BDV
genome encodes six proteins but the P24 protein was identified at
higher rates than other proteins in BDV-infected tissues.
In this study, BDV-P24 protein was constructed and subcloned into
expression plasmid pET22.
Recombinant protein expression was confirmed by SDS-PAGE and western
blotting. P24 protein was injected into rabbits with the aim of
polyclonal antibody production and immunization.
ELISA is a fast, cost-effective, and highly sensitive technique with a
lower probability of contamination compared to other diagnostic
methods. ELISA was performed to evaluate infection in
laboratory rabbits and retrospective infection was examined in 50
rabbits. The obtained results in
this study indicated that ELISA based on P24 protein has a high
potential to detect BDV infection.