10/07/1402
Prevention the formation of biofilm on orthopedic implants by melittin thin layer on chitosan/bioactive glass/vancomycin coatings
Methicillin-resistant and Vancomycin-resistant Staphylococcus aureus
bacteria (MRSA and VRSA, respectively) can seriously jeopardizes bone
implants. This research aimed to examine the potential synergistic
effects of Melittin and vancomycin in preventing MRSA and VRSA
associated bone implant infections. Chitosan/bioactive glass
nanoparticles/vancomycin composites were coated on hydrothermally etched
titanium substrates by casting method. The composite coatings were
coated by Melittin through drop casting technique. Melittin raised the
proliferation of MC3T3 cells, making it an appropriate option as
osteoinductive and antibacterial substance in coatings of orthopedic
implants. Composite coatings having combined vancomycin and Melittin
eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas
coatings containing one of them failed to kill the whole VRSA bacteria.
Therefore, chitosan/bioactive glass/vancomycin/Melittin coating can be
used as a bone implant coating because of its anti-infective properties