Investigation of the effective parameters on aptamer-based electrochemical biosensors for the detection of fumonisin B1 in maize flour
Abstract
This
study examined the effective parameters on fabrication of an aptasensor
to detect fumonisin B1 (FB1) in maize flour. For this purpose, gold
nanoparticles (AuNPs) was firstly electrodeposited onto screen printed
carbon electrode (SPCE). Then, a thiol-modified single stranded DNA
(ss-HSDNA) was immobilized on the AuNPs/SPCE electrode. By applying the
cyclic voltammetry (CV) technique, the effects of HAuCl4 and ss-HSDNA
concentrations in the electrolyte, incubation time of aptamer and FB1,
pH and temperature of the electrolyte on the peak current response were
investigated. The findings indicated that the optimal concentration of
HAuCl4 was 5 mM. The peak current of CV decreased as the concentration
of ss-HSDNA increased and the optimum ss-HSDNA concentration was chosen
at 5 µM. In addition, the CV peak currents decreased with increasing
incubation time of aptamer or FB1. The peak currents of CV first
decreased and then increased as the electrolytes temperature increased.
The electrolytes pH also showed this trend. Base on the results, this
aptasensor could be a promising tool for FB1 detection in maize flour.