In this work, a magnetic nanobiocomposite scaffold based on carboxymethylcellulose (CMC) hydrogel, silk fibroin (SF), and magnetite nanoparticles was
fabricated. The structural properties of this new magnetic
nanobiocomposite were characterized by various analyses such as FT-IR,
XRD, EDX, FE-SEM, TGA and VSM.
According to the particle size histogram, most of the particles were
between 55 and 77 nm and the value of saturation magnetization of this
nanobiocomposite was reported 41.65 emu.g− 1.
Hemolysis and MTT tests showed that the designed magnetic
nanobiocomposite was compatible with the blood. In addition, the
viability percentage of HEK293T normal cells did not change
significantly, and the proliferation rate of BT549 cancer cells
decreased in its vicinity. EC50 values for HEK293T normal cells after
48 h and 72 h were 3958 and 2566, respectively. Also, these values for
BT549 cancer cells after 48 h and 72 h were 0.4545 and 0.9967,
respectively. The efficiency of fabricated magnetic nanobiocomposite was
appraised in a magnetic fluid hyperthermia manner. The specific absorption rate (SAR) of 69 W/g (for the 1 mg/mL sample at 200 kHz) was measured under the alternating magnetic field (AMF).